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KMID : 1149220100180020251
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Korean Journal of Oriental Medical Prescription
2010 Volume.18 No. 2 p.251 ~ p.257
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Simultaneous Determination of Cinnamaldehyde and Coumarin in Oryeong-san using HPLC with Photodiode Array Detector
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Seo Chang-Seob
Shin Hyeun-Kyoo
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Abstract
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Objectives: To develop and validate High-performance liquid chromatography-photodiode array methods for simultaneous determination of two constituents in Oryeong-san(ORS).
Methods: Reverse-phase chromatography using a Gemini C18 column operating at , and photodiode array(PDA) detection at 280 nm, were used for quantification of the two marker components of ORS. The mobile phase using a gradient flow consisted of two solvent systems. Solvent A was and solvent B was acetonitrile.
Results: Calibration curves were acquired with correlation coefficient ()>0.9999, and the relative standard deviation(RSD) values(%) for intra- and inter-day precision were not exceed 1.0%. The recovery rate of each compound was in the range of 93.01-104.16%, with an RSD less than 2.0%. The contents of two compounds in ORS were 1.10-3.72 mg/g.
Conclusions : The established HPLC method will be helpful to improve quality control of ORS.
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KEYWORD
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HPLC-PAD, Oryeong-san(ORS), Simultaneous determination, Herbal formula
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